Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 15;9:132. doi: 10.1038/s41598-018-36725-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Time courses of integration by FL and truncation mutant PFV intasomes. PFV intasomes, including FL, ∆NTD, and ∆CTD, with Cy5 fluorescently labeled viral DNA were assayed for integration to a supercoiled plasmid over time. PFV FL intasomes were also assayed in the presence of 300 mM NaCl. (a) Integration products were separated by agarose gel electrophoresis and imaged for ethidium bromide (top panels) and Cy5 (bottom panels) fluorescence. (b) The ethidium bromide intensity of supercoiled plasmid is shown as the fraction of signal within each lane. The fraction of signal that is CI is the sum of the intensities of the linear band, the smear between linear and supercoiled bands, and the smear below the supercoiled band. (c) The relative Cy5 intensity within each lane is shown for the viral donor band, the linear CI product band, the HSI nicked circle band, and the smear of concerted integration products between the linear band and the viral donor. Experiments were performed at least three times with at least two independent intasome preparations. Error bars indicate standard deviation. NC, nicked circle. L-CI, linear CI products. SC, supercoiled plasmid. S-CI, smear CI products. Donor, viral donor DNA.