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. 2019 Jan 15;9:132. doi: 10.1038/s41598-018-36725-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Rescue of PFV intasome activity by additives present during preincubation. PFV FL intasomes were incubated at 37 °C for 5 min. Following the preincubation, target DNA was added and reactions were incubated for an additional 5 min at 37 °C. Additives included in the reactions were: 5 mM PCA (PCA), 0.1 mg/ml acetylated BSA (BSA), 5% DMSO (DMSO), 10% glycerol (Gly), 10% sucrose (Suc), and 10% PEG 6000 (PEG). Control reactions include no preincubation (No preinc) or no addition of an additive (−). Fluorescent products were analyzed and expressed as the fraction of fluorescent signal in each lane (bottom graph). Experiments were performed at least three times with at least two independent intasome preparations. Error bars indicate standard deviation.