Figure 7.

PFV intasome aggregation. PFV FL intasomes were incubated at 37 °C for 5 min. The reactions were then centrifuged to pellet aggregates. Pellets were analyzed by SDS-PAGE stained with Coomassie blue. (a) Reactions included 110 mM NaCl with no PCA, 300 mM NaCl with no PCA, 110 mM NaCl with 5 mM PCA, or 110 mM NaCl with 25 mM PCA. Precipitated PFV IN is expressed relative to reactions with 110 mM NaCl and no PCA. (b) FL intasomes were incubated in the presence or absence of ZnCl₂. Precipitated PFV IN is expressed relative to reactions with ZnCl₂ present. Experiments were performed at least three times with at least two independent intasome preparations. Error bars indicate standard deviation.