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. 2019 Jan 15;9:131. doi: 10.1038/s41598-018-37464-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dietary bile acid supplementation in two mouse strains, C57BL/6J (panels A–C) and 129S6/SvEvTac (panels D–F), with control (C) or high fat (H) diet and the respective versions supplemented with cholate (CC and HC). (A) Inguinal white adipose tissue (iWAT) mass after the 4 weeks feeding trial, mean ± SD. (B) Transcript abundance of uncoupling protein 1 (UCP1) in iBAT as shown in panel A, horizontal bar = mean. (C) Histological appearance of representative, hematoxylin-eosin-stained iWAT sections from the four diet groups, scale bar 50 µm. (D) Inguinal white adipose tissue (iWAT) mass after the 4 weeks feeding trial, mean ± SD. (E) Transcript abundance of uncoupling protein 1 (UCP1) in iWAT as shown in panel D, horizontal bar = mean. (F) Histological appearance of representative, hematoxylin-eosin-stained iWAT sections from the four diet groups, scale bar 50 µm. All bars are mean values ± SD; n = 6–7 (A + B) and 5–6 (D + E); results of 2-way-ANOVA in italics (A,B,D,E) with ^#significant effect, *significantly different (Sidak’s multiple testing post-hoc).