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. 2019 Jan 15;9:139. doi: 10.1038/s41598-018-37437-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Paired GSC orthotopic xenografts recapitulate the molecular and phenotypic characteristics of patient tumours. (A) Cell counting assay showing different growth kinetics of MGG70R-GSCs (70R-GSC) and MGG70RR-GSCs (70RR-GSC) in culture. Shown right are representative microscopic pictures of a neurosphere generated from a single GSC. (B) H&E staining of coronal sections of the brains bearing GSC-derived orthotopic tumours. Scale bar, 5 mm. (C) H&E staining and IHC for EGFR, phospho-EGFR, and Ki-67 (MIB-1) in orthotopic GSC xenografts. Top row, MGG70R-GSC tumours. Bottom row, MGG70RR-GSC tumours. (D) MIB-1 labeling indices and pEGFR immunopositivity (% area) of MGG70R-GSC and MGG70RR-GSC tumours. (E) FISH images of the GSC xenografts, with EGFR probe in green and CEN7 control probe in red. Left, MGG70R (pre-dacomitinib) GSC tumour. Right, MGG70RR (post-dacomitinib) GSC tumour.