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. 2019 Jan 15;9:139. doi: 10.1038/s41598-018-37437-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MGG70RR tumour and its GSC xenografts overexpress mesenchymal markers. Immunohistochemistry (IHC) for CD44 and YKL-40 in the patient tumours, MGG70R (70R) and MGG70RR (70RR), and the corresponding orthotopic GSC xenografts. Upper two rows, patient tumours. Lower two rows, xenografts. For CD44 IHC in MGG70RR-GSC tumours, a tumour-brain border area was depicted to demonstrate tumour-selective staining. Note negative staining of CD44 in tumour-associated blood vessels (arrow). Image quantification of IHC is shown on the right: upper plots, patient tumors; lower plots, xenografts.