Table 2.
Photochemical properties of caged glutamate probes.
| Caged Glu | ε (λmax) | QY | ε.QY | Time of release (ms) | 2PuCS (GM/nm) | Stability at pH 7.4 | Commercially available |
|---|---|---|---|---|---|---|---|
| CNB | 500 (350) | 0.14 | 20 | 0.021 | NR | Slow hydrolysis | Y |
| MNI | 4,500 (336) | 0.065 | 293 | NR* | 0.06 (740) | Stable | Y |
| Bhc | 17,500 (368) | 0.019 | 329 | 3–10 | 1.0 (740) | Stable | N |
| RuBi | 5,600 (450) | 0.13 | 728 | 0.05 | 0.14 (800) | Stable | Y |
| MDNI | 6,400 (330) | 0.5 | 3,200 | NR* | 0.06 (720) | Slow hydrolysis | Y |
| CDNI | 6,400 (330) | 0.5 | 3,200 | NR* | 0.06 (720) | Slow hydrolysis | N |
| DEAC450 | 43,000 (450) | 0.39 | 16,800 | NR* | 0.5 (900) | Stable frozen | N |
ε, extinction coefficient; λmax, absorption maximum; QY, quantum yield; 2PuCS, two-photon uncaging cross section; NR, not reported.
*
Biology with these p robes implies fast release.