FIGURE 2.

Replicon accumulation by differential Rep/RepA expression. Leaves of N. benthamiana were agroinfiltrated with either low (UbiF) or high (35S) expression vectors producing combinations of Rep and/or RepA, along with the replicon vector pBY-2e-NVCP. Leaf tissue samples were harvested at 4 DPI, and 1 μg of extracted total DNA was separated and visualized by ethidium bromide stained agarose gel electrophoresis. The relative intensity of replicon bands was quantified with ImageJ software. Error bars are means ± standard deviation of three or more independently infiltrated samples.