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. 2019 Jan 9;9:1974. doi: 10.3389/fpls.2018.01974

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Copyright © 2019 Diamos and Mason.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of Rep/RepA 5′ UTR mutant. Leaves of N. benthamiana were agroinfiltrated with the rituximab-producing replicon vector with (pBYe-R2-MRtx) or without (pBYR2e-MRtx) a mutated Rep/RepA 5′ UTR and analyzed after 4–5 DPI for (A) replicon band intensity quantified from 500 ng total DNA by ethidium bromide stained agarose gel or (inset) western blot. (B) Rituximab production by IgG ELISA, and (C) leaf necrosis photographed at 5 DPI. (^∗∗) Indicates p < 0.05.