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. 2019 Jan 9;9:1974. doi: 10.3389/fpls.2018.01974

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Copyright © 2019 Diamos and Mason.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Replicating vectors require lower Agrobacterium concentration for optimal expression. Leaves of N. benthamiana were agroinfiltrated with the GFP-expressing BeYDV vectors or the non-replicating vector pEAQ-HT-GFP at the indicated OD₆₀₀ values. (A) Leaf spots were assayed for GFP production by SDS-PAGE followed by quantification of fluorescence band intensity by ImageJ software. (B) Leaf images under UV light or (C) visible light. (D) Protein extractions from leaf spots agroinfiltrated at the indicated OD₆₀₀ values with a BeYDV vector expressing an HBc heterodimer were visualized by SDS-PAGE with Coomassie staining. Arrow indicates HBc heterodimer band. A representative mock-infiltrated protein extract from a different gel is shown at left for comparison. (^∗∗∗) Indicates p < 0.01.