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. 2019 Jan 9;9:3283. doi: 10.3389/fmicb.2018.03283

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Copyright © 2019 Aires, Muyzer, Serrão and Engelen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental design. (A). Five blocks along a transect with 5 m in between the blocks. (B) Example of one of the blocks showing all the different treatment plots. Between two plots within a transect there is a distance of 1 m and the distance between two plots of adjacent transects is 1.5 m. The different treatments considered in this study are colored and are as follow: Light gray: n: 0 = no nutrients, NS = no seaweeds; Dark gray: n: 2 = 250 g N m^-2 of nutrients added; Light red: n: 0 = no nutrients, Gracilaria++ = 3000 g WW m^-2 of Gracilaria added; Dark red: n: 2 = 250 g N m^-2 of nutrients added, Gracilaria++ = 3000 g WW m^-2 of Gracilaria added; Light green: n: 0 = no nutrients, Ulva++ = 3000 g WW of Ulva m^-2 added; Dark green: n: 2 = 250 g N m^-2 of nutrients added, Ulva++ = 3000 g WW of Ulva m^-2 added. Each block was composed by the same treatments and each one was sampled for each treatment totalling five replicates/treatment. No colored plots were not considered in this study.