Figure 3.

Effect of arachidonic acid, aspirin and prostaglandin on EhCP-A5 cysteine protease activity. Live control Eh were pretreated with drugs for 16 h at 37°C. (A) Arachidonic acid (100 μM), (B) aspirin (1 mM) and (C) PGE₂ (1 μM) for 16 h at 37°C. Lysates were prepared from drug treated/non-treated Eh using three cycles of freeze-thaw lysis. The bars indicate the means and the error bars indicate the standard errors of the means for three different experiments.