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. 2018 Nov 12;294(2):490–501. doi: 10.1074/jbc.RA118.006146

Figure 5.

Figure 5.

Hop2–Mnd1 binds to mixed recombinase filaments containing both Rad51 and Dmc1. A, wide-field TIRFM images of recombinase ssDNA filaments bound by 30 nm Hop2–Mnd1–mCherry (magenta) for filaments composed of 3:1 Rad51: Dmc1, 1:1 Rad51: Dmc1, and Dmc1 only, as indicated. B, distribution of integrated Hop2–Mnd1–mCherry fluorescence signal intensity values (a.u., arbitrary units) from individual ssDNA molecules for experiments conducted with Rad51 only, 3:1 Rad51:Dmc1, 1:1 Rad51:Dmc1, or Dmc1 only, as indicated. The error bars represent S.D. of the data. C, histogram showing the distribution of distances between adjacent Hop2–Mnd1–mCherry foci bound to mixed recombinase filaments composed of 3:1 Rad51:Dmc1 and 1:1 Rad51:Dmc1 ratios. The data fit a Gaussian distribution, and the mean distance between adjacent Hop2–Mnd1–mCherry foci was determined from the fit.