Figure 1.

Fatty acids contribute to Frizzled protein–protein interactions. A, crystal structure of XWnt8a in complex with mFZD8 CRD (surface representation; PDB code 4F0A) highlighting the C14 fatty acid covalently attached to XWnt8a that occupies the hydrophobic groove of mFZD8 CRD with (B) the interaction map of the fatty acid–binding site. C, crystal structure of FZD2 CRD (surface representation; tan) in complex with the FBD of TcdB (blue, surface representation; PDB code 6C0B) bridged by a C16 fatty acid (orange, space-filling model), with (D) interaction map of fatty acid–binding site. The saturation status of each fatty acid could not be determined due to the resolution of the structure. Artwork by Luciana Giono.