Figure 4.

Levels of proinflammatory gene transcripts in visceral tissue of LPS- and/or GlcN-injected adult zebrafish. Adult zebrafish were incubated with GlcN (1 g/liter of maintained water) for 12 h before abdominal GlcN (200 μg/g) injection and subsequently stimulated with LPS (100 μg/g). Visceral tissue was separated at 24 h and inos, cox-2, il-1β, il-6, tnf-α, and ifn-γ mRNA levels were determined via PCR or quantitative real-time PCR. Blots are representative of three independent experiments. All values are mean ± S.E. Asterisk denotes significantly increased from the untreated control (p < 0.05); hash mark (#) indicates significantly decreased from LPS-stimulated conditions (p < 0.05).