Figure 5.

Effects of GlcN on LPS-induced activation of MAPKs, AKT, and IκB. Lung samples were isolated from PBS or LPS (5 mg/kg)- and/or GlcN (200 mg/kg)-injected mice at 24 h. A, whole lung lysates were prepared and immunoblotted with ERK, P38, JNK, AKT, p-ERK, p-P38, p-JNK, and p-AKT antibodies. Relative densitometric intensities for P-ERK, P-JNK1, and P-AKT were quantitatively measured by normalizing to ERK, P38, JNK, and AKT levels, respectively. B and C, representative confocal immunofluorescence staining images of P-IκBα and p-P65 are shown. Nuclei were counterstained with DAPI for visualization in lung tissue (B). Scale bar, 500 μm. Positive staining for p-IκBα and p-P65 was quantitatively assessed (C). D, Western blot analysis of p-IκBα, IκBα, and GAPDH proteins from whole lung lysates. Blots and fluorescent images are representative of three independent experiments. All values are presented as mean ± S.E. Asterisk denotes significantly increased from the untreated control (p < 0.05); hash mark (#) indicates significantly decreased from LPS-stimulated conditions (p < 0.05).