Figure 5.

Activity of the JH I geometric isomers in vivo. A, capacity of the native S-(E,E)-JH I, its stereoisomers, and deoxy-JH I to induce ectopic expression of Kr-h1 mRNA in Drosophila pupae. Animals were treated at the white puparium stage and collected 24 h later. Shown are normalized mean qRT-PCR data from four biological replicates, each comprising three individual pupae for each compound. The mRNA levels are plotted on a logarithmic scale as -fold increase relative to treatment with solvent (acetone) alone for which the value was arbitrarily set to 1. Error bars represent S.D. Different letters above the data indicate that activity of the individual compounds differed significantly (p < 0.05) as determined by one-way analysis of variance of log₁₀-transformed data with Tukey's multiple post hoc comparison. B, effect of the JH I stereoisomers and deoxy-JH I on the ability of flies to complete development. Animals were again treated with the compounds as white puparia (n = 15–20 specimens per treatment).