Table 1.

Binding affinities of JH ligands to the Gce protein and Gibbs (ΔG) and ligand strain energies

Compound	Affinity of ligand binding to Gce (Ki)a	ΔG of ligand binding	Ligand strain energy
	m	kcal mol−1	kcal mol−1
R-JH III	1.10 × 10−8b	−108.70	8.44
S-(E,E)-JH Ic	1.38 × 10−8	−137.80	11.16
S-(E,Z)-JH I	4.47 × 10−7	−130.42	9.72
R-(E,Z)-JH I	4.79 × 10−7	−122.24	17.88
S-(Z,E)-JH I	6.14 × 10−7	−119.60	21.41
R-(Z,E)-JH I	2.40 × 10−6	−109.10	25.18
S-(Z,Z)-JH I	2.26 × 10−5	−106.65	24.22
R-(Z,Z)-JH I	2.89 × 10−5	−100.16	24.52

^a Determined from competition against R,S-[³H]JH III binding to the Gce protein.

^b K_i determined for the racemic mixture of 10R and 10S enantiomers of (2E,6E)-JH III.

^c Native hormone with the absolute configuration 10R,11S-(2E,6E)-JH I.