Figure 3.

In vivo effect of cystamine injection on the circulating hemocyte number. A, relative GC count, B, relative SGC count, and C, relative THC count at 1, 3, 6, 24, and 48 h post-injection of cystamine (75 μg cystamine/g (■) and 150 μg cystamine/g (▴)) or CPBS (●) as a control. Four to 12 crayfish were used in each experimental group. The data points represent the mean from four to 12 crayfish, and the error bar represents the S.D. value. *, p < 0.05, **, p < 0.01, and ***, p < 0.001 indicate a significant difference compared with the control.