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Cell-free production and purification of EhFNT and BtFdhC. A and B show detergent screens with C-terminal GFP fusions of EhFNT (first 90 amino acids truncated) and BtFdhC. C, Ni2+-affinity purification of full-length EhFNT (without GFP) via a C-terminal His6 tag. The elution fractions contained partially SDS-resistant EhFNT dimers and oligomers.
