Table 3.
Efficiency of translation of mutations from maize variant D2-124 to EPSPS from other crop species
The 18 mutations present in maize variant D2–124 were mapped onto the amino acid sequence of the predicted mature form of EPSPS from the species shown (see “Results” for details). Proteins were expressed, purified and evaluated as described under “Experimental procedures” and the legend to Table I.
| Speciesa | kglyb | kcat | Km PEP | Ki |
|---|---|---|---|---|
| min−1 | μm | |||
| Maize | 175 ± 2.5 | 631 ± 5 | 18.1 ± 0.7 | 893 ± 53 |
| Rice | 174 ± 3.1 | 707 ± 17 | 22.3 ± 2.7 | 893 ± 89 |
| Sorghum | 172 ± 8.5 | 846 ± 2.9 | 20.7 ± 1.7 | 404 ± 44 |
| Sunflower | 155 ± 7.7 | 381 ± 7.0 | 18.0 ± 1.8 | 918 ± 37 |
| Grapevine | 144 ± 11 | 652 ± 8.1 | 30.4 ± 1.7 | 1150 ± 98 |
| Cotton | 143 ± 8.4 | 413 ± 2.7 | 15.3 ± 0.6 | 940 ± 85 |
| Cassava | 133 ± 6.3 | 688 ± 8.6 | 24.7 ± 1.5 | 1400 ± 228 |
| Soybean | 114 ± 4.1 | 618 ± 4.8 | 90.3 ± 2.3 | 3550 ± 374 |
| Wheat | 102 ± 6.1 | 223 ± 4.3 | 18.9 ± 1.7 | 820 ± 119 |
a Species, accession: Z. mays, CAA44974.1; Oryza sativa, AF413082.1; Sorghum bicolor, XM_002436379.2; H. annuus, XM_022161807.1; Vitis vinifera, NC_012021.3; Gossypium hirsutum, UniProt A7Y7Y2; Manihot esculenta, XM_021758443.1; Glycine max, XM_003516991.3; T. aestivum, ACH72672.1.
b kgly is the enzyme turnover (min−1) at 30 μm PEP and S3P, 1 mm glyphosate (see “Results” for rational as a fitness parameter).