Table 2.

Primer sets for PCR analysis of gene expression

Genes	Primers
Mouse 18S ribosome
Forward	5′-CGGCTACCACATCCAAGGAA-3′
Reverse	5′-GCTGGAATTACCGCGGCT-3′
Mouse Sox2
Forward	5′-CCCACCTACAGCATGTCCTAC-3′
Reverse	5′-GCCTCGGACTTGACCACAG-3′
Mouse Pou5f1
Forward	5′-TGAGCCGTCTTTCCACCAGGC-3′
Reverse	5′-GGAAGCTTAGCCAGGTTCGAGGATC-3′
Mouse Nanog
Forward	5′-TTTATTGGTGCCAGAGCAAACC-3′
Reverse	5′-GTCTCCAAAGCCTAGAGTTAAC-3′
Mouse c-Myc
Forward	5′-ATGCCCCTCAACGTGAACTTC-3′
Reverse	5′-CGCAACATAGGATGGAGAGCA-3′
Mouse Klf4
Forward	5′-GGCGAGTCTGACATGGCTG-3′
Reverse	5′-GCTGGACGCAGTGTCTTCTC-3′
Mouse β-actin
Forward	5′-ACTGTCGAGTCGCGTCC-3′
Reverse	5′-CGCAGCGATATCGTCATCCAT-3′
Mouse Aldh3a1
Forward	5′-AGAAGCCCCTGGCACTCTAT-3′
Reverse	5′-GCAAAGTGGGCACAGTGATG-3′