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. 2019 Jan 10;176(1-2):306–317.e16. doi: 10.1016/j.cell.2018.10.041

Figure 5.

Figure 5

Oligopeptide Mixtures Promote Stumpy Formation In Vitro

(A) Growth of pleomorphic or monomorphic T. brucei cells in varying concentrations of autoclaved brain heart infusion broth at 48 hr. Error bars, SEM.

(B) PAD1 expression of pleomorphic T. brucei cells in varying concentrations of autoclaved brain heart infusion broth at 48h. Error bars, SEM.

(C) Representative images of PAD1 expression and morphology of pleomorphic cells in varying concentrations of BHI broth at 48 hr. PAD1 expression (in green) is evident on increasing proportions of the parasites with higher concentrations of autoclaved BHI; these cells also appear stumpy in morphology. The parasite nucleus and kinetoplast (stained with DAPI) is pseudo colored in magenta. Bar, 25 μm.

(D) Growth of pleomorphic T. brucei in vitro in the presence of different oligopeptide containing extracts expressed relative to their growth without extract (“control”) at 48 hr. Error bars, SEM.

(E) PAD1 expression of pleomorphic T. brucei exposed to the different concentrations of oligopeptide containing extracts at 48 hr. Error bars, SEM.