Table 1.
Identification of Top* PREOP and POD2 Proteins from iTRAQ
| Concordance Rate (%) | Detection Rate (%) | No. of Up-regulated | No. of Down- regulated | |
|---|---|---|---|---|
| PREOP | ||||
| Histidine-rich glycoprotein (HRG) | 88 | 67 | 7 | 1 |
| Alpha-1B-glycoprotein (A1BG) | 88 | 67 | 1 | 7 |
| Fibrinogen alpha chain (FIBA) | 88 | 67 | 1 | 7 |
| Zinc-alpha-2-glycoprotein (AZGP1) | 83 | 100 | 2 | 10 |
| Complement C2 (CO2) | 80 | 42 | 1 | 4 |
| Fibrinogen beta chain (FIBB) | 80 | 42 | 1 | 4 |
| Hemopexin (HPX) | 78 | 75 | 2 | 7 |
| Complement component C9 (CO9) | 75 | 67 | 6 | 2 |
| ITIH2 | 75 | 67 | 6 | 2 |
| Antithrombin-III (ANT3) | 75 | 67 | 2 | 6 |
| Retinol-binding protein 4 (RET4) | 75 | 67 | 2 | 6 |
| IGFALS | 75 | 67 | 2 | 6 |
| Serotransferrin (TRFE) | 75 | 33 | 3 | 1 |
| Complement C4-B (CO4B) | 75 | 33 | 3 | 1 |
| Thyroxine-binding globulin (THBG) | 75 | 33 | 3 | 1 |
| Extracellular matrix protein 1 (ECM1) | 75 | 33 | 1 | 3 |
| Complement factor I (CFAI) | 67 | 75 | 3 | 6 |
| C-reactive protein (CRP) | 67 | 50 | 4 | 2 |
| CBPN | 67 | 50 | 2 | 4 |
| POD2 | ||||
| CRP | 100 | 50 | 6 | 0 |
| Haptoglobin (HP) | 100 | 42 | 0 | 5 |
| ECM1 | 100 | 33 | 0 | 4 |
| Plasminogen (PLMN) | 88 | 67 | 7 | 1 |
| Apolipoprotein A-IV (APOA4) | 80 | 83 | 2 | 8 |
| Alpha-1-acid glycoprotein 1 (ORM1) | 80 | 42 | 4 | 1 |
| HPX | 78 | 75 | 2 | 7 |
| Clusterin (CLUS) | 75 | 100 | 9 | 3 |
| AZGP1 | 75 | 100 | 3 | 9 |
| HRG | 75 | 67 | 6 | 2 |
| Fibrinogen alpha chain (FIBA) | 75 | 67 | 6 | 2 |
| CO4B | 75 | 33 | 1 | 3 |
| Complement factor D (CFAD) | 75 | 33 | 1 | 3 |
| TRFE | 75 | 33 | 1 | 3 |
| Complement C4-A (CO4A) | 75 | 33 | 1 | 3 |
| Hyaluronan-binding protein 2 (HABP2) | 75 | 33 | 3 | 1 |
| Carboxypeptidase B2 (CBPB2) | 72 | 58 | 2 | 5 |
| IBP3 | 70 | 83 | 3 | 7 |
| Alpha-1-antichymotrypsin (SERPINA3) | 67 | 100 | 8 | 4 |
Notes: CBPN = carboxypeptidase N catalytic chain; IBP3 = insulin-like growth factor-binding protein 3; IGFALS = insulin-like growth factor-binding protein complex acid labile subunit; ITIH2 = inter-alpha-trypsin inhibitor heavy chain H2; iTRAQ = isobaric tag for relative and absolute quantitation; POD2 = postoperative day 2; PREOP = preoperative. N = 24 (12 matched pairs). No. of upregulated and downregulated are dependent on the detection rate. Proteins highlighted in the dark gray indicate the four selected proteins for ELISA confirmation that appear in both the PREOP and POD2 lists. Proteins highlighted in light gray indicate the three selected proteins for ELISA confirmation that appear only in the POD2 list.
*Top proteins were defined as proteins with (i) detection rate >1/3 (33.3%) and (ii) concordance rate >2/3 (66.7%).