Table 1.
Summary of cell lines reported as supporting C. parvum replication. Where multiple cell lines are listed, subsequent data refers to those in bold.
| Cell Line/Type | Oocyst Source | Media | Time Maintained | Life Cycle | Reference(s) |
|---|---|---|---|---|---|
| Chorioallantoic membrane of chicken embryos (Hubbard broiler and White Leghorn breed) | Naturally infected calf, infected AIDS patient | N/A | 8 days | Complete life cycle | Current and Long (1983) |
| Human foetal lung, Porcine kidney, Primary chicken kidney | AIDS patient | Minimal Essential Media with Earle's Salts, 2% FBS | 7 days | Complete life cycle | Current and Haynes (1984) |
| HT29.74, Clone of colorectal cell line HT29 | AIDS patient | RPMI-1640, 10% FBS, 24 mM sodium bicarbonate, 1 mM sodium pyruvate, 20 mM HEPES (for undifferentiated cells), Leibovitz L-15 medium containing 5 mM galactose, 6 mM pyruvate, 1 mM L-glutamine, 20 mM HEPES, antibiotics, 10% dialyzed fetal calf serum (for differentiated cells) | 13 days, rapid loss of reinfection after 5 days | Asexual stages only | Flanigan et al. (1991) |
| Mouse peritoneal macrophages | Naturally infected calves | RPMI-1640, 10% inactivated FBS | 3 days | Asexual, few instances of gamonts or oocysts | Martinez et al. (1992) |
| RL95-2, derived from human endometrial carcinoma | Experimentally infected calves | Dulbecco's modified Eagle's medium and Ham's F12 medium (1:1 ratio), 10% FBS, 10 mM HEPES, 5 μg bovine insulin, 2% NaHCO3 (w/v) | 4 days | Complete life cycle | (Rasmussen et al., 1993) |
| Madin-Darby canine kidney (MDCK) | Infected calves | Minimal Essential Media, 10% FBS | 5 days | Complete life cycle | Rosales et al. (1993) |
| HRT-18 (Human rectal tumour) | Experimentally infected calves | RPMI-1640, 10% Inactivated FBS | 6 days | Asexual stages only | (Woodmansee and Joachim, 1983) |
| Caco-2 (Colorectal adenocarcinoma) | Experimentally infected goats and lambs, AIDS patient with persistent cryptosporidiosis | N/A | 5 days | Complete life cycle | (Buraud et al., 1991) |
| Madin-Darby bovine kidney (MDBK) | Naturally infected calves (Villacorta et al., 1996, Upton et al., 1994a,b) | Minimal Essential Media, 26 mM NaHCO3, 4% FBS (Villacorta), RPMI-1640 with L-glutamine, 10% FBS, 26 mM NaHCO3, 15 mM HEPES (Upton) | 3 days | Complete life cycle | (Upton et al., 1994a,b; Villacorta et al., 1996) |
| BALB-3T3, BT-549, Hs700t, HT-1080, RL95-2, HCT-8 (Colorectal adenocarcinoma) | Infected cows | RPMI-1640 with L-glutamine, 10% FBS | 3 days | Not described | (Upton et al., 1994a,b) |
| MRC-5 (lung fibroblast) | Clinical isolate | Medium 199, Earles salts; essential amino acids, L-glutamine, 0.075% w/v sodium bicarbonate, 10% FBS | 5 days | Not described | (Dawson et al., 2004) |
| BS-C-1 (African green monkey kidney cells) | Infected calves | Minimal Essential Media, 2 mM L-glutamine, 10% FBS | 3 days | Complete life cycle | (Qi Deng and Cliver, 1998) |
| COLO-680N | Commercial source (infected cows) | RPMI-1640 with L-glutamine, 10% FBS | 8 weeks | Complete life cycle | (Miller et al., 2017) |