Fig. 5.

Ginsenoside CK does not enhance cell death in HEK-hP2X4 cells. HEK-293 cells stably expressing hP2X4 or hP2X7 (50,000 cells/well) were plated into 96-well plates for 24 hours prior to stimulation with ATP. Cells were pretreated with antagonists AZ10606120 (AZ) or 5-BDBD for 10 minutes before ATP was added. Cells were exposed to the indicated concentrations of ATP or ATP + CK (10 μM) for 24 hours, and viability was measured using MTS absorbance. MTS was added for the final 4 hours of incubation, and absorbance read using a Flexstation 3 reader. Data were normalized to cells treated with vehicle control and are expressed as percentage of control. Data are from four independent experiments.