Figure 4.

(A) ROS generation analyses of drug-loaded HBNCs in DPBS. DCFH-DA (5 μM) was added to each drug suspension, followed by a red light irradiation (632 nm, 15 mW/cm²) for 0–120 min. The concentration of RB was fixed at 40 μM. (B) ROS generation of RB in the presence of a serial concentration of bPEI triggered by 20-min light irradiation. (C) Light-induced RB release from co-drug loaded HBNCs in DPBS (10% FBS). An aliquot of sample was exposed to a red light for 0–6 h and the drug released at different time point was collected and measured by centrifugation at 40,000 g for 20 min at 4°C. (D) Hydrodynamic size changes of PTX-HBNCs and RB/PTX-HBNCs in DPBS (1% BSA) followed by light exposure (+) or darkness (–) of 6 h.