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For the competitive binding studies, free HA was employed as a competitor. Both of the (a) (RB/PTX-BNCs)- and (b) (RB/PTX-HBNCs)-treated cells were co-incubated with or without free HA (10 mg/mL) in culture medium for 4 h. Cellular uptake of RB/PTX-HBNCs was analyzed by using (A) microscopy and (B) flow cytometry. Scale bar: 50 μm. Statistical significance at a level of *p < 0.05.
