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Epidermal cells' confocal images of Arabidopsis cotyledons, transiently co-expressing RFP::NIP1;1 with (A–C) GFP::SYP51 after 48 h of co-expression; (D–F) GFP::SYP51 after 72 h; (G–I) Golgi-independent vacuolar marker GFPgl133Chi after 48 h. The first column shows GFP signal in green, the second column shows the RFP signal in magenta, whereas the third column shows the overlay of fluorescent signals and co-localization in white. Scale bar = 20 μm.
