Figure 1.

Deletion of MyD88 in Intestinal Mesenchymal Cells Reduces Tumorigenesis in the Apc^min/+ Model of Sporadic Intestinal Cancer

(A and B) Total number of tumors per mouse (A) and number of tumors per intestinal part (B) in 4-month-old Apc^min/+Myd88^IMCko mice (n = 15) and their littermate controls (n = 18). The insert shows the number of colonic tumors.

(C and D) Size of small intestinal tumors presented as mean tumor size (C) and distribution of tumors per size (D) in the two genotypes.

(E) Representative BrdU and Tunel staining in small intestinal tumors of Apc^min/+Myd88^IMCko mice and their littermate controls. DAPI was used to stain the nuclei in the Tunel stainings.

(F and G) Quantification of the number of BrdU⁺ cells per tumor (F) and Tunel⁺ cells (G) per field in equal-sized tumors (n = 6 mice per genotype).

(H) Number of dysplastic lesions per mouse in 6-week-old Apc^min/+Myd88^IMCko and their littermate controls (n = 6–7 mice per genotype).

(I) Number of tumors per mouse in Myd88^IMCko mice (n = 8) and their littermate controls (n = 7) at the end of the AOM/DSS protocol (one representative experiment of four performed).

Data represent mean ± SEM. ^∗∗p < 0.01, ^∗∗∗p < 0.001, ns = not significant. Scale bars represent 50 μm. See also Figures S1 and S2.