REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
His-affinity tag, mouse monoclonal	Takara Bio	Cat#631212
XRCC1 Antibody, rabbit polyclonal	Bio-techne	Cat#NBP1-87154
HRP-conjugated mouse anti-mouse IgG	GE healthcare	Cat#NA931
Bacterial and Virus Strains
NEB 5-alpha Competent E. coli	New England Biolabs	Cat#C2987H
BL21(DE3) Competent E. coli	New England Biolabs	Cat#C2527I
Chemicals, Peptides, and Recombinant Proteins
[15N] NH4Cl	CortecNet	Cat#CN80P10
[13C3] glycerol	CortecNet	Cat#CC1065P10
Isopropyl β-D-1-thiogalactopyranoside	Generon	Cat#Gen-S-02122
HEPES	Fisher Scientific	Cat#10081113
NaCl	Fisher Scientific	Cat#10735921
Imidazole	Acros Organics	Cat#301870010
TCEP	Sigma-Aldrich	Cat#646547
cOmplete, EDTA-free Protease Inhibitor Cocktail	Sigma-Aldrich	Cat#4693159001
Talon resin	TaKaRa Bio	Cat#635503
Calf thymus histones	Sigma-Aldrich	Cat#H9250
Tris-HCl	Fisher Scientific	Cat#10316893
MgCl2	Fisher Scientific	Cat#10386743
NAD+	Sigma-Aldrich	Cat#N8410
trichloroacetic acid	Fisher Scientific	Cat#10775151
EDTA	Fisher Scientific	Cat#10716481
KOH	Fisher Scientific	Cat#10448990
Ammonium acetate	Fisher Scientific	Cat#10365260
Guanidine HCl	Acros Organics	Cat#120230025
3-Aminophenylboronic acid monohydrate	Sigma-Aldrich	Cat#287512
Bio-Rex 70 Cation Exchange Resin	Bio-Rad	Cat#1425822
Urea	Fisher Scientific	Cat#10578260
Bromophenol blue	Fisher Scientific	Cat#10679733
Xylene cyanol	Sigma-Aldrich	Cat#X4126
Pierce Color Silver Stain Kit	Fisher Scientific	Cat#10096113
D2O	Sigma-Aldrich	Cat#151882
6% DNA Retardation Gel	Fisher Scientific	Cat#12080086
Tris-borate-EDTA	Fisher Scientific	Cat#10542985
Gibson Assembly	New England Biolabs	Cat#E2611L
human PARP1	Trevigen	Cat#4668-02K-01
Tween 20	Sigma-Aldrich	Cat#P9416
3,3′,5,5′-tetramethylbenzidine	Sigma-Aldrich	Cat#T4319
Triton X-100	Sigma-Aldrich	Cat#T9284
Dulbecco’s Phosphate-Buffered Saline (PBS)	ThermoFisher Scientific	Cat#14190136
Hoechst 34580	Sigma-Aldrich	Cat#63493
Dulbecco’s modified Eagle’s medium (DMEM)	ThermoFisher Scientific	Cat#21969035
Foetal bovine serum	Sigma-Aldrich	Cat#F7524
Glutamine	ThermoFisher Scientific	Cat#25030081
Penicillin-Streptomycin	Sigma-Aldrich	Cat#P4333
Genejuice	Novagen	Cat#70967
Blasticidin	InvivoGen	Cat#ant-bl-1
Methyl methanesulfonate	Sigma-Aldrich	Cat#129925
Low-gelling-temperature agarose, Type VII-A	Sigma-Aldrich	Cat#A0701
DMSO	Sigma-Aldrich	Cat#276855
SybrGreen I	Fisher Scientific	Cat#S7563
Hydrogen peroxide	Sigma-Aldrich	Cat#H1009
Paraformaldehyde	Agar Scientific	Cat#AGR1026
Hoechst 33342	Sigma-Aldrich	Cat#B2261
ECL reagent	Fisher Scientific	Cat#10455145
PD MidiTrap G-10 column	Sigma-Aldrich	Cat#GE28-9180
Tankyrase 1	(Elliott et al., 2015)	N/A
Deposited Data
Biological Magnetic Resonance Bank		27598
Experimental Models: Cell Lines
Osteosarcoma U2-OS	Genome Damage and Stability Centre cell repository	ID: U2-OS
Oligonucleotides
Oligonucleotides for DNA-binding experiments, see Figure S1.	Integrated DNA Technologies	N/A
Recombinant DNA
pET15b	Novagen	69661
peGFP-N1	Clontech	6085-1
pET15b-SUMO-XRCC1-BRCT1	This paper	N/A
Software and Algorithms
GraphPad Prism7 for Mac OS X	Graphpad	https://www.graphpad.com/scientific-software/prism/
SlideBook 6	3i	https://www.intelligent-imaging.com/slidebook
CcpNmr Analysis	Collaborative Computing Project for NMR	https://www.ccpn.ac.uk/
Comet Assay IV software	Perceptive Instruments	http://www.scorecomets.com/comet-scoring/comet-assay-iv
Harmony high-content analysis software	PerkinElmer	Cat#HH17000001
ImageJ64	ImageJ Software	https://imagej.nih.gov/ij/
Other
BD FACSMelody	BD Biosciences	N/A
Operetta CLS high-content analysis system	PerkinElmer	Cat#HH16000000
3i Spinning Disk Confocal microscope	3i	N/A