Figure 7.

Progressive cerebral microvascular occlusions in rTg-DI rats. A: Twelve-month–old brain sections from rTg-DI rats were stained for hemosiderin to identify microhemorrhages (blue) and counterstained with pararosaniline (pink) in the thalamus. B: Adjacent tissue sections were stained for calcium (black) and counterstained with pararosaniline (pink) in the thalamus. C: Lower magnification revealed that the occluded, calcified microvessels were restricted to the thalamic region. D: The number of thalamic microvessel occlusions was counted in progressively aged rTg-DI rats. Numerous occluded capillaries/microvessels were observed around microbleeds specifically in the thalamic region of rTg-DI rats. Data are expressed as means ± SD. n = 5 to 6 rTg-DI rats per group (D). Scale bars: 50 μm (A and B); 200 μm (C).