Figure 2.

Phenotypic and molecular characterization of skin equivalents. (a) Spontaneous blistering occurred only in uncorrected keratinocyte‐derived skin equivalents derived from a patient with epidermolysis bullosa simplex (red arrows). Scale bars = 1 cm. hKc, human keratinocyte. (b) Azure–methylene blue–basic fuchsin‐stained resin sections of explants show the junction between murine (mm, left) and human (hSE, right) tissue. Scale bars = 100 μm. (c) C7 expression at the dermoepidermal junction (DEJ) is visible on explanted skin sections and respective controls (white arrows), but not in mouse tissue (d). Scale bars = 20 μm. (e) Skin sections derived from EBDM‐1+RTM (RNA trans‐splicing molecule) transplants show trans‐splicing‐derived green fluorescence in the human skin equivalent part, barely and unspecifically detectable in murine tissue (f). Scale bars = 20 μm. Dotted lines show the DEJ.