Figure 2.

Global metabolic profiling of feces from germ free (GF) and conventionally raised (CONV-R) mice determined by ¹H NMR. O-PLS-DA scores represent indicative power of models, p-value (CV-ANOVA) demonstrates the significance testing of OPLS model. Correlation coefficientcoded loadings plots for the models (right) from NMR spectra fecal extracts displaying changes and significance. The upward-pointed peak indicates the metabolite that peak represents (metabolite labeled) presented in higher level in GF mice. Peak pointing downward represents the metabolite presented more in CONV-R mice. The color-coded correlation coefficient indicates the significance of that change, the significance increases, as color gets warmer. A cutoff value of |r| > 0.754 (r > 0.754 and r < −0.754) is chosen for correlation coefficient as significant based on the discrimination significance (p ≤ 0.05).