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. 2019 Jan 2;2019:9720546. doi: 10.1155/2019/9720546

Figure 5.

Figure 5

The effects of vitamin C at 0.5 mM on the number of DA neurons in 15-day-old flies. (a) Immunostaining images of brains from control (TH-Control) and dUCH knockdown flies (TH-dUCH KD) using anti-TH antibody. PPM1/2 and PPM3 clusters are enclosed by white dashed boxes and shown with higher magnifications in lower panels (A5–A8 and A9–A12, respectively). Scale bars indicate 100 µm (A1–A4), 20 µm (A5–A8), and 10 µm (A9–A12). (b) Quantification of dopaminergic neurons in control and dUCH knockdown flies at 15 days. One-way ANOVA with Tukey's test, p < 0.05 and ∗∗ p < 0.01 and ∗∗∗ p < 0.001, n=10, and error bars indicate the standard deviation of data. TH-Control (+; +; TH-GAL4/+) and TH-dUCH KD (+; +; TH-GAL4/UAS-dUCH.IR).