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. 2019 Jan 9;9:3021. doi: 10.3389/fimmu.2018.03021

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Copyright © 2019 Bianconi, Alcalá-Franco, Scarselli, Dalsass, Buccato, Colaprico, Marchi, Masignani and Bragonzi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic workflow of antigen selection in silico. The complete PAO1 proteome has been analyzed as described in the text. Distribution of ORFs according to predicted sub-cellular localization using Psort is summarized at the top. Below, selection steps succession is reported, indicating the number of candidates (hits) selected after each step. C: cytoplasm; IN: inner membrane; P: periplasm; OM: outer membrane.