Fig. 4.

Effects of depletion or addition of IL-8 combined with GEN on stemness of SKOV3 cells induced by Co-CM. SKOV3 cells were treated with conditioned medium from THP-1 macrophages and were treated with depletion or addition of IL-8 Co-CM in the presence or absence of GEN. The sphere and colony formation rate (a and b, scale bar, 100 μm) and expression of CD133 and CD44 (c) in SKOV3 cells induced by Co-CM in depletion of IL-8 and GEN alone or in combination were shown. The sphere and colony formation rate (d and e, scale bar, 100 μm) as well as expression of CD133 and CD44 (f) in SKOV3 cells induced by Co-CM by adding IL-8 and GEN alone or in combination were shown. *P < 0.05, vs SKOV3 cells treated with Co-CM. ^#P < 0.05, vs SKOV3 cells treated with the conditioned medium obtained from GEN (10.0 μM) treatment. These experiments were performed at least three times