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. 2019 Jan 15;38:19. doi: 10.1186/s13046-018-1010-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Overexpression of STAT3 rescued the effects of depletion of IL-8 combined with GEN on stemness of SKOV3 cells induced by Co-CM. SKOV3 cells were treated with IL-8 depleting Co-CM from SKOV3-derived OCSLCs co-cultured with THP-1 macrophages transduced with adenovirus expressing STAT3 and in the presence or absence of GEN. Ad-STAT3: The cells transduced with adenovirus expressing STAT3. The sphere and colony formation rate (a and b, scale bar, 100 μm) as well as expression of CD133 and CD44 (c) in SKOV3 cells induced by Co-CM were shown. *P < 0.05, vs treatment with depletion of IL-8. ^#P < 0.05, vs co-treatment with depletion of IL-8 and GEN (10.0 μM). These experiments were performed at least thrice