Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 15;10:28. doi: 10.1186/s13287-018-1120-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 2 — OCT4 increased proliferation capacity and differentiation potential in hHFMSCs. Cell proliferation curve (a) and cell population doubling time (b) of hHFMSCs^EGFP and hHFMSCs^OCT4. c Clone formation assay of hHFMSCs^EGFP and hHFMSCs^OCT4; the enlarged views show the difference between the two cell clones. The histogram is the formation rate for each clone (bar, 200 μm). d Representative sphere images and bar graph of the percentages of spheres in hHFMSCs^EGFP and hHFMSCs^OCT. e Western blot results for the expression of proliferation-associated proteins PCNA and cyclin D1 in hHFMSCs^EGFP and hHFMSCs^OCT4. f Immunofluorescence of proliferation-associated protein Ki67 expression and location in hHFMSCs^EGFP and hHFMSCs^OCT4 (bar, 50 μm). g Effects of OCT4 on hHFMSC cell cycle phase distribution. The percentage of the G1, G2, and S phase in the cell cycle (left of the lower panel) and the PI (right of the lower panel) of the hHFMSCs^EGFP and hHFMSCs^OCT4. “dip” is the abbreviation for diploid. h Osteogenic differentiation in hHFMSCs^EGFP and hHFMSCs^OCT4. Calcium nodules were detected by Alizarin Red S staining (bar, 200 μm). (*p < 0.05; **p < 0.01; ***p < 0.001)