Figure 4.

Infection kinetic of Guinardia delicatula RCC3083 by GdelRNAV-01. Abundances of diatom hosts in the control culture (black circles) and in infected cultures (open triangles) were obtained using optical microscopy. Viral titers (open hexagons) were estimated using the MPN method. Error bars were estimated based on counts obtained in triplicates of infected cultures. Gray rectangles represent the dark phases. Pictures obtained using transmitted-light and epifluorescence microscopy illustrate the morphology of G. delicatula cells in control and infected cultures at T0 and Tfinal. With epifluorescence microscopy the red natural fluorescence of chloroplasts and green fluorescence of PicoGreen stained nucleic acids are observed. At Tfinal, the green fluorescence is due to the presence of bacteria. Scale bars: 20 μm.