Figure 2.

ChromograninA positive hormone-negative (CPHN) cells do not replicate during fetal and infant life. Representative pancreatic sections from fetal (A) and infant (B) donors immunostained for Endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, and ghrelin) (white), chromograninA (green), Ki67 (red), and DAPI (blue). Yellow arrows indicate CPHN cells. CPHN cells were rarely positive for Ki67 staining in both fetal and infant groups; no detectable difference was found in the frequency of replicative CPHN cells between fetal and infant pancreatic sections. Scale bars: 100 μm for low power and 50 μm for high magnification images.