Fig. 1. Cr(vi)-Induced cytotoxicity in L-02 hepatocytes. L-02 hepatocytes were exposed to different concentrations of Cr(vi) (0, 5, 10, and 15 μM) for 24 h. (A) Cell viability was determined by MTT assay. (B) AST and ALT levels were examined using the commercial kits. (C) PTP opening rate was determined using the commercial kit. (D) MMP was assayed with the cationic fluorescent dye Rh123. (E) The protein levels of AIF, Cyt C, cleaved Caspase-9 and cleaved Caspase-3 were examined using western blotting. *p < 0.05, compared with control.