Fig. 3. CLU was up-regulated in Cr(vi)-exposed L-02 hepatocytes. L-02 hepatocytes were exposed to different concentrations of Cr(vi) (0, 5, 10, and 15 μM) for 24 h. (A) CLU mRNA expression was determined using qPCR. The data were analyzed by the 2^–ΔΔCt method. (B) CLU protein level was examined using western blotting. The hepatocytes were exposed to 15 μM Cr(vi) for 24 h with or without MitoTEMPO pretreatment (10 μM for 1 h). (C) The protein expression levels of ATM, IGF-1, and CLU were determined. *p < 0.05, compared with control.