Fig. 4. CLU may regulate MRCC I activity. Human L-02 hepatocytes and two HCC cell lines HepG2 and HCCLM3 were used. (A) CLU protein level was examined using western blotting. (B) MRCC I activity was determined using the commercial kit. The hepatocytes were transfected either with CLU-cDNA (EV-transfected cells served as a control) or with CLU shRNA (Scr-transfected cells served as a control). (C) CLU protein level. (D) MRCC I activity. (E) NDUFS3 protein level was examined using western blotting. *p < 0.05, compared with control.