Fig. 5. CLU-induced increase of MRCC I activity protected against Cr(vi)-induced cytotoxicity. L-02 EV and OE cells were exposed to different concentrations of Cr(vi) (0, 5, 10, and 15 μM) for 24 h. (A) MRCC I activity was determined using the commercial kit. (B) ROS level was measured using HE. (C) PTP opening rate was determined using the commercial kit. (D) MMP was assayed with the cationic fluorescent dye Rh123. (E) The protein expression levels of AIF, Cyt C, cleaved Caspase-9 and cleaved Caspase-3 were examined using western blotting. (F) Cell viability was determined by MTT assay. (G) AST and ALT levels were examined using the commercial kits. *p < 0.05, compared with control. ^#p < 0.05, compared with L-02 EV hepatocytes.