Figure 6.

MB‐3 treatment of TNGA mouse embryonic stem cells results in increased transcriptional heterogeneity from key pluripotency and differentiation regulatory loci. (A): T‐distributed stochastic neighbor embedding (t‐SNE) plot of transcriptional profiles of individual TNGA cells treated with DMSO or MB‐3 in ESLIF and 2i conditions. (B): Overall changes in gene expression squared coefficient of variation between DMSO and MB‐3‐treated cells assayed from ESLIF (left) and 2i (right) culture conditions (paired Student's t test, p < .0001). (C): Ordered representation of gene expression changes between DMSO and MB‐3‐treated TNGA cells in ESLIF (top) and 2i (bottom) conditions. Mean expression represented as dot, standard error of the mean is represented as vertical bars. Abbreviation: DMSO, dimethyl sulfoxide.