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. 2019 Jan 9;8:661. doi: 10.3389/fonc.2018.00661

Figure 2.

Figure 2

PDCD4 knockdown suppressed Rb and p-Rb and modulated the expression of CDKs. PDCD4 knockdown suppressed the expression of Rb, CDK1, CDK2, CDK4, CDK6, and Rb phosphorylation in (A) HepG2 (wild-type p53) and (B) Huh7 (mutant p53 and p16-deficient). (C) The expression of CDK1 significantly up-regulated while the modulations of CDK2, CDK4, and CDK6 were not significant in Hep3B (p53- and Rb-deficient) cells. Protein extracts from untreated control cells (ctr), negative control siRNA-treated cells (nc) and PDCD4-specific p2 siRNA-treated (p2) hepatoma cells were analyzed by Western blotting at 24, 48, and 72 h of culture after siRNA treatment using specific antibodies against the components indicated in the figures. These experiments were independently repeated three times, and similar results were obtained. Each band was determined and normalized to β-actin. The data in (D) HepG2, (E) Huh7, and (F) Hep3B represent the mean ± SD obtained from the respective experiments. The t-test shows the p-value between negative control siRNA and PDCD4-specific siRNA treated cells at 72 h of culture.