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. 2019 Jan 9;8:661. doi: 10.3389/fonc.2018.00661

Figure 4.

Figure 4

The p27 (Cip/Kip) expression was changed only slightly or down-regulated in HepG2 and Huh7 but was up-regulated in Hep3B cells by p2 siRNA-mediated PDCD4 knockdown(A) The hepatoma cells HepG2, Huh7, and Hep3B were treated with negative control siRNA (nc) or PDCD4-specific p2 siRNA (p2) and then subjected to a Western blot analysis using p27-, PDCD4-, and β-actin-specific antibodies at the culture time indicated in the figures. ctr, control cells without siRNA treatment. The experiments were repeated at least three times, and similar results were obtained each time. The data in the diagrams represent the mean ± SD obtained from the experiments. t-test: * p < 0.05. →, the p27 band was identified by p27 knockdown using a specific siRNA. (B) A real time RT-PCR analysis of p27 in PDCD4 knockdown HepG2, Huh7, and Hep3B cells. The cells were treated with negative control siRNA (nc) or PDCD4-specific p2 siRNA (p2) and cultured for 48 h. The experiments were repeated independently 3 times. Data were expressed as the mean ± SD obtained from the experiments. t-test: *p < 0.05.