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. 2019 Mar;14(3):532–541. doi: 10.4103/1673-5374.245480

Figure 1.

Figure 1

Effect of DFO on hindlimb function of rats with SCI.

(A) Experimental design. Thirty minutes before spinal cord contusion injury, rats received DFO (100 mg/kg) or saline. DFO was injected daily for 7 days. Transmission electron microscopy was conducted on the injured tissue at 1 and 24 hours post injury. Western blot assay was conducted at 2 and 7 days post injury. Real-time quantitative polymerase chain reaction was conducted at 1 hour, 8 hours, and 3 days post injury. HE staining and immunohistology were conducted at 2 and 8 weeks post injury. BBB score was assessed every week for 8 weeks. (B) The degree of hindlimb recovery was assessed at 1, 2, 4, and 8 weeks after SCI using the BBB score. *P < 0.05, **P < 0.01, vs. SCI group. Data are expressed as the mean ± SEM (n = 3; two-way analysis of variance followed by Tukey’s post hoc test). TEM: Transmission electron microscope; ACSF2: Acyl-CoA synthetase family member 2; BBB: Basso, Beattie and Bresnahan locomotor rating scale; DFO: deferoxamine; GFAP: glial fibrillary acidic protein; GPX4: glutathione peroxidase 4; GSH: glutathione; HE: hematoxylin eosin; HNE: 4-hydroxynonenal; IREB2: iron-responsive element-binding protein 2; NeuN: neuronal nuclear antigen; SCI: spinal cord injury; TEM: Transmission electron microscope; xCT: system Xc- light chain; min: minutes; h: hour(s); d: days; w: week(s).