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. 2019 Mar;14(3):501–505. doi: 10.4103/1673-5374.245476

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Copyright: © Neural Regeneration Research

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Direct conversion of human fibroblasts into neurons by nucleofection of non-viral vectors.

(A) The three combinations of episomal vectors: pCXLE-Ascl1-shP53 (AP), pCXLE-Ascl1-miR124 (AM) and pCXLE-Ascl1-miR124-shP53 (AMP). (B, C) The protocol of human foreskin fibroblast cell conversion into neurons. FM: Full medium. (D) Cell morphology during fibroblast reprogramming in the AMP group. Day 2: fibroblast cell morphology; Day 4: the cells gradually changed their morphological characteristics; Day 11: the shape of the cells exhibited neuron-like morphology. Scale bars: 50 μm.